in vitro assays Search Results


purexpress in vitro protein synthesis kit  (New England Biolabs)
96
New England Biolabs purexpress in vitro protein synthesis kit
Purexpress In Vitro Protein Synthesis Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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la pcr in vitro cloning kit  (TaKaRa)
94
TaKaRa la pcr in vitro cloning kit
La Pcr In Vitro Cloning Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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la pcr in vitro cloning kit - by Bioz Stars, 2026-03
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purexpress  (New England Biolabs)
96
New England Biolabs purexpress
Purexpress, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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electrodes  (KCAS Bioanalytical and Biomarker Services)
94
KCAS Bioanalytical and Biomarker Services electrodes
Electrodes, supplied by KCAS Bioanalytical and Biomarker Services, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vitro transcription kit  (TaKaRa)
95
TaKaRa vitro transcription kit
In vitro assay to evaluate cleavage efficiency by the Guide-it sgRNA screening kit. ( a ) PCR products from the amplification of EgFAD2 (lane 1: sgRNA1, lane 2: sgRNA2) and EgPAT (lane 3: sgRNA3, lane 4: sgRNA4) sgRNA templates at 130 bp for in vitro <t>transcription.</t> ( b ) PCR products are amplified from oil palm genomic DNA containing sgRNA target sites; the PCR templates of candidate sgRNAs are then in vitro combined with a recombinant Cas9 for a fragment cleavage site ( c ). EgFAD2-T1 (lane 1), EgFAD2-T2 (lane 2), EgPAT-T1 (lane 3), and EgPAT-T2 (lane 4) represent the PCR fragments with 921 bp, 986 bp, 903 bp, and 978 bp length, respectively. Cleavage fragments were present for all sgRNAs; they were cleaved into two fragments by the sgRNA/Cas9 complex: 503 bp and 395 bp for EgFAD2-T1; 559 bp and 404 bp for EgFAD2-T2; 531 bp and 349 bp for EgPAT-T1; and 287 bp and 668 bp for EgPAT-T2. Lane C is a control fragment that has a size of 614 bp and cleaved DNA of 350 bp and 264 bp. Lane W is a negative control, and lane M is a 1 kb plus DNA marker (Invitrogen)
Vitro Transcription Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
vitro transcription kit - by Bioz Stars, 2026-03
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colorimetric hmg coa reductase activity assay kit  (Danaher Inc)
94
Danaher Inc colorimetric hmg coa reductase activity assay kit
In vitro assay to evaluate cleavage efficiency by the Guide-it sgRNA screening kit. ( a ) PCR products from the amplification of EgFAD2 (lane 1: sgRNA1, lane 2: sgRNA2) and EgPAT (lane 3: sgRNA3, lane 4: sgRNA4) sgRNA templates at 130 bp for in vitro <t>transcription.</t> ( b ) PCR products are amplified from oil palm genomic DNA containing sgRNA target sites; the PCR templates of candidate sgRNAs are then in vitro combined with a recombinant Cas9 for a fragment cleavage site ( c ). EgFAD2-T1 (lane 1), EgFAD2-T2 (lane 2), EgPAT-T1 (lane 3), and EgPAT-T2 (lane 4) represent the PCR fragments with 921 bp, 986 bp, 903 bp, and 978 bp length, respectively. Cleavage fragments were present for all sgRNAs; they were cleaved into two fragments by the sgRNA/Cas9 complex: 503 bp and 395 bp for EgFAD2-T1; 559 bp and 404 bp for EgFAD2-T2; 531 bp and 349 bp for EgPAT-T1; and 287 bp and 668 bp for EgPAT-T2. Lane C is a control fragment that has a size of 614 bp and cleaved DNA of 350 bp and 264 bp. Lane W is a negative control, and lane M is a 1 kb plus DNA marker (Invitrogen)
Colorimetric Hmg Coa Reductase Activity Assay Kit, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vitro translation  (Jena Bioscience)
93
Jena Bioscience vitro translation
In vitro assay to evaluate cleavage efficiency by the Guide-it sgRNA screening kit. ( a ) PCR products from the amplification of EgFAD2 (lane 1: sgRNA1, lane 2: sgRNA2) and EgPAT (lane 3: sgRNA3, lane 4: sgRNA4) sgRNA templates at 130 bp for in vitro <t>transcription.</t> ( b ) PCR products are amplified from oil palm genomic DNA containing sgRNA target sites; the PCR templates of candidate sgRNAs are then in vitro combined with a recombinant Cas9 for a fragment cleavage site ( c ). EgFAD2-T1 (lane 1), EgFAD2-T2 (lane 2), EgPAT-T1 (lane 3), and EgPAT-T2 (lane 4) represent the PCR fragments with 921 bp, 986 bp, 903 bp, and 978 bp length, respectively. Cleavage fragments were present for all sgRNAs; they were cleaved into two fragments by the sgRNA/Cas9 complex: 503 bp and 395 bp for EgFAD2-T1; 559 bp and 404 bp for EgFAD2-T2; 531 bp and 349 bp for EgPAT-T1; and 287 bp and 668 bp for EgPAT-T2. Lane C is a control fragment that has a size of 614 bp and cleaved DNA of 350 bp and 264 bp. Lane W is a negative control, and lane M is a 1 kb plus DNA marker (Invitrogen)
Vitro Translation, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vitro angiogenesis assay  (R&D Systems)
93
R&D Systems vitro angiogenesis assay
MicroRNA biogenesis is essential for endothelial cell tumor formation. EOMA cell stable transfectants were generated using lentiviral shRNA delivery. A and B, targeted post-transcriptional gene silencing of dicer was confirmed by Western blot (A) and real time PCR (B). C and D, Matrigel <t>angiogenesis</t> assay was used to evaluate the functional effects of dicer knockdown in vitro. Cells were stained with calcein-AM (C), and the area within the formed tubes was quantitated by analyzing three high powered fields per well using AxioVision Rel 4.8 software (D). Matrigel tube formation was compromised in dicer knockdown EOMA cells. E, reduced tumor size in response to dicer knockdown. F, incidence of tumor formation in mice injected with EOMA cells transfected with controls shRNA or dicer shRNA. G, tumor volume as quantified using calipers (length × width × height). The results are means ± S.D. of at least three independent experiments. *, p < 0.05.
Vitro Angiogenesis Assay, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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liquid chromatography  (KCAS Bioanalytical and Biomarker Services)
93
KCAS Bioanalytical and Biomarker Services liquid chromatography
MicroRNA biogenesis is essential for endothelial cell tumor formation. EOMA cell stable transfectants were generated using lentiviral shRNA delivery. A and B, targeted post-transcriptional gene silencing of dicer was confirmed by Western blot (A) and real time PCR (B). C and D, Matrigel <t>angiogenesis</t> assay was used to evaluate the functional effects of dicer knockdown in vitro. Cells were stained with calcein-AM (C), and the area within the formed tubes was quantitated by analyzing three high powered fields per well using AxioVision Rel 4.8 software (D). Matrigel tube formation was compromised in dicer knockdown EOMA cells. E, reduced tumor size in response to dicer knockdown. F, incidence of tumor formation in mice injected with EOMA cells transfected with controls shRNA or dicer shRNA. G, tumor volume as quantified using calipers (length × width × height). The results are means ± S.D. of at least three independent experiments. *, p < 0.05.
Liquid Chromatography, supplied by KCAS Bioanalytical and Biomarker Services, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vitro angiogenesis assay kit  (Trevigen)
94
Trevigen vitro angiogenesis assay kit
MicroRNA biogenesis is essential for endothelial cell tumor formation. EOMA cell stable transfectants were generated using lentiviral shRNA delivery. A and B, targeted post-transcriptional gene silencing of dicer was confirmed by Western blot (A) and real time PCR (B). C and D, Matrigel <t>angiogenesis</t> assay was used to evaluate the functional effects of dicer knockdown in vitro. Cells were stained with calcein-AM (C), and the area within the formed tubes was quantitated by analyzing three high powered fields per well using AxioVision Rel 4.8 software (D). Matrigel tube formation was compromised in dicer knockdown EOMA cells. E, reduced tumor size in response to dicer knockdown. F, incidence of tumor formation in mice injected with EOMA cells transfected with controls shRNA or dicer shRNA. G, tumor volume as quantified using calipers (length × width × height). The results are means ± S.D. of at least three independent experiments. *, p < 0.05.
Vitro Angiogenesis Assay Kit, supplied by Trevigen, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hep 2 slides  (Antibodies Inc)
90
Antibodies Inc hep 2 slides
MicroRNA biogenesis is essential for endothelial cell tumor formation. EOMA cell stable transfectants were generated using lentiviral shRNA delivery. A and B, targeted post-transcriptional gene silencing of dicer was confirmed by Western blot (A) and real time PCR (B). C and D, Matrigel <t>angiogenesis</t> assay was used to evaluate the functional effects of dicer knockdown in vitro. Cells were stained with calcein-AM (C), and the area within the formed tubes was quantitated by analyzing three high powered fields per well using AxioVision Rel 4.8 software (D). Matrigel tube formation was compromised in dicer knockdown EOMA cells. E, reduced tumor size in response to dicer knockdown. F, incidence of tumor formation in mice injected with EOMA cells transfected with controls shRNA or dicer shRNA. G, tumor volume as quantified using calipers (length × width × height). The results are means ± S.D. of at least three independent experiments. *, p < 0.05.
Hep 2 Slides, supplied by Antibodies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lexsy in vitro translation kit jena bioscience  (Jena Bioscience)
93
Jena Bioscience lexsy in vitro translation kit jena bioscience
MicroRNA biogenesis is essential for endothelial cell tumor formation. EOMA cell stable transfectants were generated using lentiviral shRNA delivery. A and B, targeted post-transcriptional gene silencing of dicer was confirmed by Western blot (A) and real time PCR (B). C and D, Matrigel <t>angiogenesis</t> assay was used to evaluate the functional effects of dicer knockdown in vitro. Cells were stained with calcein-AM (C), and the area within the formed tubes was quantitated by analyzing three high powered fields per well using AxioVision Rel 4.8 software (D). Matrigel tube formation was compromised in dicer knockdown EOMA cells. E, reduced tumor size in response to dicer knockdown. F, incidence of tumor formation in mice injected with EOMA cells transfected with controls shRNA or dicer shRNA. G, tumor volume as quantified using calipers (length × width × height). The results are means ± S.D. of at least three independent experiments. *, p < 0.05.
Lexsy In Vitro Translation Kit Jena Bioscience, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


In vitro assay to evaluate cleavage efficiency by the Guide-it sgRNA screening kit. ( a ) PCR products from the amplification of EgFAD2 (lane 1: sgRNA1, lane 2: sgRNA2) and EgPAT (lane 3: sgRNA3, lane 4: sgRNA4) sgRNA templates at 130 bp for in vitro transcription. ( b ) PCR products are amplified from oil palm genomic DNA containing sgRNA target sites; the PCR templates of candidate sgRNAs are then in vitro combined with a recombinant Cas9 for a fragment cleavage site ( c ). EgFAD2-T1 (lane 1), EgFAD2-T2 (lane 2), EgPAT-T1 (lane 3), and EgPAT-T2 (lane 4) represent the PCR fragments with 921 bp, 986 bp, 903 bp, and 978 bp length, respectively. Cleavage fragments were present for all sgRNAs; they were cleaved into two fragments by the sgRNA/Cas9 complex: 503 bp and 395 bp for EgFAD2-T1; 559 bp and 404 bp for EgFAD2-T2; 531 bp and 349 bp for EgPAT-T1; and 287 bp and 668 bp for EgPAT-T2. Lane C is a control fragment that has a size of 614 bp and cleaved DNA of 350 bp and 264 bp. Lane W is a negative control, and lane M is a 1 kb plus DNA marker (Invitrogen)

Journal: Journal of Genetic Engineering & Biotechnology

Article Title: Multiplex CRISPR/Cas9 gene-editing platform in oil palm targeting mutations in EgFAD2 and EgPAT genes

doi: 10.1186/s43141-022-00459-5

Figure Lengend Snippet: In vitro assay to evaluate cleavage efficiency by the Guide-it sgRNA screening kit. ( a ) PCR products from the amplification of EgFAD2 (lane 1: sgRNA1, lane 2: sgRNA2) and EgPAT (lane 3: sgRNA3, lane 4: sgRNA4) sgRNA templates at 130 bp for in vitro transcription. ( b ) PCR products are amplified from oil palm genomic DNA containing sgRNA target sites; the PCR templates of candidate sgRNAs are then in vitro combined with a recombinant Cas9 for a fragment cleavage site ( c ). EgFAD2-T1 (lane 1), EgFAD2-T2 (lane 2), EgPAT-T1 (lane 3), and EgPAT-T2 (lane 4) represent the PCR fragments with 921 bp, 986 bp, 903 bp, and 978 bp length, respectively. Cleavage fragments were present for all sgRNAs; they were cleaved into two fragments by the sgRNA/Cas9 complex: 503 bp and 395 bp for EgFAD2-T1; 559 bp and 404 bp for EgFAD2-T2; 531 bp and 349 bp for EgPAT-T1; and 287 bp and 668 bp for EgPAT-T2. Lane C is a control fragment that has a size of 614 bp and cleaved DNA of 350 bp and 264 bp. Lane W is a negative control, and lane M is a 1 kb plus DNA marker (Invitrogen)

Article Snippet: The sgRNAs designed for targeting the EgFAD2 and EgPAT genes were evaluated based on target cleavage efficiency using the Guide-it™ sgRNA In Vitro Transcription kit and the Guide-it™ Screening Systems Kit (Takara Bio Inc.) according to the manufacturer’s instructions.

Techniques: In Vitro, Amplification, Recombinant, Control, Negative Control, Marker

MicroRNA biogenesis is essential for endothelial cell tumor formation. EOMA cell stable transfectants were generated using lentiviral shRNA delivery. A and B, targeted post-transcriptional gene silencing of dicer was confirmed by Western blot (A) and real time PCR (B). C and D, Matrigel angiogenesis assay was used to evaluate the functional effects of dicer knockdown in vitro. Cells were stained with calcein-AM (C), and the area within the formed tubes was quantitated by analyzing three high powered fields per well using AxioVision Rel 4.8 software (D). Matrigel tube formation was compromised in dicer knockdown EOMA cells. E, reduced tumor size in response to dicer knockdown. F, incidence of tumor formation in mice injected with EOMA cells transfected with controls shRNA or dicer shRNA. G, tumor volume as quantified using calipers (length × width × height). The results are means ± S.D. of at least three independent experiments. *, p < 0.05.

Journal: The Journal of Biological Chemistry

Article Title: Dicer Knockdown Inhibits Endothelial Cell Tumor Growth via MicroRNA 21a-3p Targeting of Nox-4 *

doi: 10.1074/jbc.M113.519264

Figure Lengend Snippet: MicroRNA biogenesis is essential for endothelial cell tumor formation. EOMA cell stable transfectants were generated using lentiviral shRNA delivery. A and B, targeted post-transcriptional gene silencing of dicer was confirmed by Western blot (A) and real time PCR (B). C and D, Matrigel angiogenesis assay was used to evaluate the functional effects of dicer knockdown in vitro. Cells were stained with calcein-AM (C), and the area within the formed tubes was quantitated by analyzing three high powered fields per well using AxioVision Rel 4.8 software (D). Matrigel tube formation was compromised in dicer knockdown EOMA cells. E, reduced tumor size in response to dicer knockdown. F, incidence of tumor formation in mice injected with EOMA cells transfected with controls shRNA or dicer shRNA. G, tumor volume as quantified using calipers (length × width × height). The results are means ± S.D. of at least three independent experiments. *, p < 0.05.

Article Snippet: In Vitro Angiogenesis Assay Four-well plates were coated with 100 μl of Matrigel® (Cultrex® Basement membrane extract reduced growth factor; R&D Systems, Minneapolis, MN) and let to solidify for 30 min at 37 °C.

Techniques: Generated, shRNA, Western Blot, Real-time Polymerase Chain Reaction, Angiogenesis Assay, Functional Assay, In Vitro, Staining, Software, Injection, Transfection